› 8eo1

X-ray diffraction
1.28Å resolution

Human PU.1 ETS-Domain (165-270) Bound to d(AATAAGCGGAAGTGGG) with Hemi-methylated CpG (reverse strand)

Released:
DOI: 10.2210/pdb8eo1/pdb
PDB entry 8eo1 coloured by chain, front view.
PDB entry 8eo1 coloured by chain, side view.
PDB entry 8eo1 coloured by chain, top view.
Source organisms:
Primary publication:
DNA selection by the master transcription factor PU.1.
Terrell JR, Taylor SJ, Schneider AL, Lu Y, Vernon TN, Xhani S, Gumpper RH, Luo M, Wilson WD, Steidl U, Poon GMK
Cell Rep 42 112671 (2023)
PMID: 37352101

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-220426 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor PU.1 Chain: F
Molecule details ›
Chain: F
Length: 106 amino acids
Theoretical weight: 12.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17947 (Residues: 165-270; Coverage: 39%)
Gene name: SPI1
Sequence domains: Ets-domain
DNA (5'-D(*AP*AP*TP*AP*AP*GP*CP*GP*GP*AP*AP*GP*TP*GP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 5.04 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*CP*CP*CP*AP*CP*TP*TP*CP*GP*CP*TP*TP*AP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.77 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
1 modified residue:
Ligand 5CM 1 x 5CM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 42.993Å b: 60.649Å c: 44.451Å
α: 90° β: 116.722° γ: 90°
R-values:
R R work R free
0.149 0.148 0.18
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 mention without citation

DNA selection by the master transcription factor PU.1.
Terrell et al. (2023)