› 8eqj

Electron Microscopy
3Å resolution

Structure of SARS-CoV-2 Orf3a in late endosome/lysosome-like membrane environment, MSP1D1 nanodisc

Released:
DOI: 10.2210/pdb8eqj/pdb
PDB entry 8eqj coloured by chain, front view.
PDB entry 8eqj coloured by chain, side view.
PDB entry 8eqj coloured by chain, top view.
Source organism: Severe acute respiratory syndrome coronavirus 2
Primary publication:
The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins.
Miller AN, Houlihan PR, Matamala E, Cabezas-Bratesco D, Lee GY, Cristofori-Armstrong B, Dilan TL, Sanchez-Martinez S, Matthies D, Yan R, Yu Z, Ren D, Brauchi SE, Clapham DE
OpenAccess logo Elife 12 (2023)
PMID: 36695574
Related structures: EMD-28538

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORF3a protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 36.49 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC3 (Residues: 1-275; Coverage: 100%)
Gene name: 3a
Sequence domains: Betacoronavirus viroporin

Ligands and Environments

1 bound ligand:
Ligand PEE 4 x PEE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-28538
Expression system: Homo sapiens

Quick links

Citations

2 review citations

To Be or Not to Be an Ion Channel: Cryo-EM Structures Have a Say.
Chen et al. (2023)
1 more

1 mention without citation

SARS-CoV-2 ORF3a-Mediated NF-κB Activation Is Not Dependent on TRAF-Binding Sequence.
Busscher et al. (2023)