› 8eqq

X-ray diffraction
2.13Å resolution

Crystal structure of E.coli DsbA mutant E37A

Released:
DOI: 10.2210/pdb8eqq/pdb
PDB entry 8eqq coloured by chain, front view.
PDB entry 8eqq coloured by chain, side view.
PDB entry 8eqq coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A Buried Water Network Modulates the Activity of the Escherichia coli Disulphide Catalyst DsbA.
Wang G, Qin J, Verderosa AD, Hor L, Santos-Martin C, Paxman JJ, Martin JL, Totsika M, Heras B
OpenAccess logo Antioxidants (Basel) 12 (2023)
PMID: 36829940

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol:disulfide interchange protein DsbA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 189 amino acids
Theoretical weight: 21.1 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEG4 (Residues: 20-208; Coverage: 100%)
Gene names: JW3832, b3860, dsbA, dsf, ppfA
Sequence domains: DSBA-like thioredoxin domain

Ligands and Environments

1 bound ligand:
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 61.84Å b: 81.067Å c: 105.247Å
α: 90° β: 93.64° γ: 90°
R-values:
R R work R free
0.186 0.184 0.231
Expression system: Escherichia coli BL21(DE3)

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