› 8eqv

Electron Microscopy
3.64Å resolution

Cryo-EM structure of PRC2 in complex with the long isoform of AEBP2

Released:
DOI: 10.2210/pdb8eqv/pdb
PDB entry 8eqv coloured by chain, front view.
PDB entry 8eqv coloured by chain, side view.
PDB entry 8eqv coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Boudes M, Zhang Q, Flanigan SF, Davidovich C
Related structures: EMD-28547

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-271845 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Histone-binding protein RBBP4 Chain: A
Molecule details ›
Chain: A
Length: 425 amino acids
Theoretical weight: 47.71 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q09028 (Residues: 1-425; Coverage: 100%)
Gene names: RBAP48, RBBP4
Sequence domains:
Zinc finger protein AEBP2 Chain: C
Molecule details ›
Chain: C
Length: 517 amino acids
Theoretical weight: 54.54 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6ZN18 (Residues: 1-517; Coverage: 100%)
Gene name: AEBP2
Polycomb protein EED Chain: E
Molecule details ›
Chain: E
Length: 441 amino acids
Theoretical weight: 50.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O75530 (Residues: 1-441; Coverage: 100%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Histone-lysine N-methyltransferase EZH2 Chain: D
Molecule details ›
Chain: D
Length: 746 amino acids
Theoretical weight: 85.49 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q15910 (Residues: 1-746; Coverage: 100%)
Gene names: EZH2, KMT6
Sequence domains:
Polycomb protein SUZ12 Chain: B
Molecule details ›
Chain: B
Length: 739 amino acids
Theoretical weight: 83.18 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q15022 (Residues: 1-739; Coverage: 100%)
Gene names: CHET9, JJAZ1, KIAA0160, SUZ12
Sequence domains: VEFS-Box of polycomb protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.64Å
Relevant EMDB volumes: EMD-28547
Expression system: Trichoplusia ni

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