› 8erp

Electron Microscopy
3.7Å resolution

Structure of Xenopus cholinephosphotransferase1 in complex with CDP-choline

Released:
DOI: 10.2210/pdb8erp/pdb
PDB entry 8erp coloured by chain, front view.
PDB entry 8erp coloured by chain, side view.
PDB entry 8erp coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
Structure of a eukaryotic cholinephosphotransferase-1 reveals mechanisms of substrate recognition and catalysis.
Wang L, Zhou M
OpenAccess logo Nat Commun 14 2753 (2023)
PMID: 37179328
Related structures: EMD-28557

Function and Biology Details

Reaction catalysed: 
CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215234 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholinephosphotransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 402 amino acids
Theoretical weight: 44.54 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q4KLV1 (Residues: 1-402; Coverage: 100%)
Gene name: chpt1
Sequence domains: CDP-alcohol phosphatidyltransferase

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand LBN 20 x LBN
Ligand CDC 2 x CDC
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-28557
Expression system: Spodoptera frugiperda

Quick links

Citations

1 citations in other articles

Architects of the membrane: structures of eukaryotic choline phosphotransferase 1 and choline/ethanolamine phosphotransferase 1.
Raina et al. (2023)