› 8ete

X-ray diffraction
2.3Å resolution

Bile Salt Hydrolase from B. longum with covalent inhibitor bound

Released:
DOI: 10.2210/pdb8ete/pdb
PDB entry 8ete coloured by chain, front view.
PDB entry 8ete coloured by chain, side view.
PDB entry 8ete coloured by chain, top view.
Source organism: Bifidobacterium longum
Entry authors: Walker ME, Redinbo MR

Function and Biology Details

Reactions catalysed: 
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H(2)O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine
Chenodeoxycholoyltaurine + H(2)O = chenodeoxycholate + taurine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bile salt hydrolase/transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.19 KDa
Source organism: Bifidobacterium longum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KK62 (Residues: 1-317; Coverage: 100%)
Gene name: bsh
Sequence domains: Linear amide C-N hydrolases, choloylglycine hydrolase family

Ligands and Environments

1 bound ligand:
Ligand WU5 2 x WU5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3221
Unit cell:
a: 100.085Å b: 100.085Å c: 165.187Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.251 0.284
Expression system: Escherichia coli

Quick links