› 8etl

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed: 
Glc(3)Man(9)GlcNAc(2)-[protein] + H(2)O = Glc(2)Man(9)GlcNAc(2)-[protein] + beta-D-glucopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannosyl-oligosaccharide glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 819 amino acids
Theoretical weight: 93.32 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Homo sapiens
UniProt:
  • Canonical: G0SFD1 (Residues: 30-809; Coverage: 97%)
Gene name: CTHT_0061620
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand VND 2 x VND
Ligand GOL 3 x GOL
Ligand SO4 13 x SO4
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 135.598Å b: 178.525Å c: 180.299Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.232
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Expanding horizons of iminosugars as broad-spectrum anti-virals: mechanism, efficacy and novel developments.
Liu et al. (2024)
2 other articles

1 mention without citation

Structure-Based Design of Potent Iminosugar Inhibitors of Endoplasmic Reticulum α-Glucosidase I with Anti-SARS-CoV-2 Activity.
Karade et al. (2023)