› 8ew3

Electron Microscopy
2.65Å resolution

Cryo EM structure of Vibrio cholerae NQR

Released:
DOI: 10.2210/pdb8ew3/pdb
PDB entry 8ew3 coloured by chain, front view.
PDB entry 8ew3 coloured by chain, side view.
PDB entry 8ew3 coloured by chain, top view.
Source organism: Vibrio cholerae O395
Entry authors: Fuller JR, Juarez O
Related structures: EMD-28641

Function and Biology Details

Reaction catalysed: 
NADH + ubiquinone + n Na(+)(Side 1) = NAD(+) + ubiquinol + n Na(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107567 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Na(+)-translocating NADH-quinone reductase subunit A Chain: A
Molecule details ›
Chain: A
Length: 446 amino acids
Theoretical weight: 48.68 KDa
Source organism: Vibrio cholerae O395
Expression system: Vibrio cholerae O395
UniProt:
  • Canonical: A5F5X1 (Residues: 1-446; Coverage: 100%)
Gene names: VC0395_A1884, VC395_2411, nqrA
Sequence domains:
Na(+)-translocating NADH-quinone reductase subunit B Chain: B
Molecule details ›
Chain: B
Length: 415 amino acids
Theoretical weight: 45.39 KDa
Source organism: Vibrio cholerae O395
Expression system: Vibrio cholerae O395
UniProt:
  • Canonical: A5F5X0 (Residues: 1-415; Coverage: 100%)
Gene names: VC0395_A1883, VC395_2410, nqrB
Sequence domains: NQR2, RnfD, RnfE family
Na(+)-translocating NADH-quinone reductase subunit C Chain: C
Molecule details ›
Chain: C
Length: 257 amino acids
Theoretical weight: 27.65 KDa
Source organism: Vibrio cholerae O395
Expression system: Vibrio cholerae O395
UniProt:
  • Canonical: A5F5Y7 (Residues: 1-257; Coverage: 100%)
Gene names: VC0395_A1882, VC395_2409, nqrC
Sequence domains: FMN-binding domain
Na(+)-translocating NADH-quinone reductase subunit D Chain: D
Molecule details ›
Chain: D
Length: 210 amino acids
Theoretical weight: 22.85 KDa
Source organism: Vibrio cholerae O395
Expression system: Vibrio cholerae O395
UniProt:
  • Canonical: A5F5Y6 (Residues: 1-210; Coverage: 100%)
Gene names: VC0395_A1881, VC395_2408, nqrD
Sequence domains: Rnf-Nqr subunit, membrane protein
Na(+)-translocating NADH-quinone reductase subunit E Chain: E
Molecule details ›
Chain: E
Length: 198 amino acids
Theoretical weight: 21.48 KDa
Source organism: Vibrio cholerae O395
Expression system: Vibrio cholerae O395
UniProt:
  • Canonical: A5F5Y5 (Residues: 1-198; Coverage: 100%)
Gene names: VC0395_A1880, VC395_2407, nqrE
Sequence domains: Rnf-Nqr subunit, membrane protein
Na(+)-translocating NADH-quinone reductase subunit F Chain: F
Molecule details ›
Chain: F
Length: 408 amino acids
Theoretical weight: 45.11 KDa
Source organism: Vibrio cholerae O395
Expression system: Vibrio cholerae O395
UniProt:
  • Canonical: A5F5Y4 (Residues: 1-408; Coverage: 100%)
Gene names: VC0395_A1879, VC395_2406, nqrF
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand FMN 2 x FMN
Ligand RBF 1 x RBF
Ligand UQ1 1 x UQ1
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.652Å
Relevant EMDB volumes: EMD-28641
Expression system: Vibrio cholerae O395

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