› 8ewg

Electron Microscopy
2.9Å resolution

Cryo-EM structure of a riboendonclease

Released:
DOI: 10.2210/pdb8ewg/pdb
PDB entry 8ewg coloured by chain, front view.
PDB entry 8ewg coloured by chain, side view.
PDB entry 8ewg coloured by chain, top view.
Source organism: Thermoclostridium caenicola
Primary publication:
Structural Basis for the Ribonuclease Activity of a Thermostable CRISPR-Cas13a from Thermoclostridium caenicola.
Wang F, Zhang C, Xu H, Zeng W, Ma L, Li Z
J Mol Biol 435 168197 (2023)
PMID: 37442412
Related structures: EMD-28645

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227508 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endoribonuclease Cas13a Chain: A
Molecule details ›
Chain: A
Length: 1225 amino acids
Theoretical weight: 143.58 KDa
Source organism: Thermoclostridium caenicola
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1M6GDI0 (Residues: 1-1225; Coverage: 100%)
Gene name: SAMN05444373_102315
RNA (56-MER) Chain: B
Molecule details ›
Chain: B
Length: 60 nucleotides
Theoretical weight: 19.3 KDa
Source organism: Thermoclostridium caenicola
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-28645
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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