› 8ewo

X-ray diffraction
2.47Å resolution

Crystal structure of putative glyoxylase II from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb8ewo/pdb
PDB entry 8ewo coloured by chain, front view.
PDB entry 8ewo coloured by chain, side view.
PDB entry 8ewo coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Stogios PJ, Skarina T, Endres M, Savchenko A, Joachimiak A, Satchell KJF, Center for Structural Genomics of Infectious Diseases (CSGID), Center for Structural Biology of Infectious Diseases (CSBID)

Function and Biology Details

Reaction catalysed: 
S-(2-hydroxyacyl)glutathione + H(2)O = glutathione + a 2-hydroxy carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxyacylglutathione hydrolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 259 amino acids
Theoretical weight: 28.96 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I2T1 (Residues: 1-258; Coverage: 100%)
Gene names: PA1813, gloB
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand ZN 6 x ZN
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 70.723Å b: 76.608Å c: 75.43Å
α: 90° β: 94.63° γ: 90°
R-values:
R R work R free
0.181 0.179 0.219
Expression system: Escherichia coli BL21(DE3)

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