› 8ewt

X-ray diffraction
2.03Å resolution

Bile salt hydrolase A from Lactobacillus gasseri bound to covalent probe

Released:
DOI: 10.2210/pdb8ewt/pdb
PDB entry 8ewt coloured by chain, front view.
PDB entry 8ewt coloured by chain, side view.
PDB entry 8ewt coloured by chain, top view.
Source organism: Lactobacillus gasseri
Entry authors: Walker ME, Redinbo MR

Function and Biology Details

Reactions catalysed: 
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H(2)O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine
Chenodeoxycholoyltaurine + H(2)O = chenodeoxycholate + taurine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Conjugated bile acid hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 35.86 KDa
Source organism: Lactobacillus gasseri
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A805Z5R7 (Residues: 1-316; Coverage: 100%)
Gene names: LGAS_0054, bsh
Sequence domains: Linear amide C-N hydrolases, choloylglycine hydrolase family

Ligands and Environments

2 bound ligands:
Ligand X3I 2 x X3I
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P43212
Unit cell:
a: 108.44Å b: 108.44Å c: 161.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.226
Expression system: Escherichia coli

Quick links