› 8eww

X-ray diffraction
2.8Å resolution

Structure of Arabidopsis fatty acid amide hydrolase mutant S305A

Released:
DOI: 10.2210/pdb8eww/pdb
PDB entry 8eww coloured by chain, front view.
PDB entry 8eww coloured by chain, side view.
PDB entry 8eww coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Aziz M, Wang X, Gaguancela OA, Chapman KD

Function and Biology Details

Reaction catalysed: 
Anandamide + H(2)O = arachidonic acid + ethanolamine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid amide hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 636 amino acids
Theoretical weight: 69.56 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7XJJ7 (Residues: 1-607; Coverage: 100%)
Gene names: At5g64440, FAAH, T12B11.3
Sequence domains: Amidase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P21
Unit cell:
a: 72.451Å b: 81.324Å c: 133.18Å
α: 90° β: 104.51° γ: 90°
R-values:
R R work R free
0.236 0.234 0.26
Expression system: Escherichia coli

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