8exa

Electron Microscopy
3.14Å resolution

ISDra2 TnpB in complex with reRNA and cognate DNA, conformation 1 (RuvC domain resolved)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-214629 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
RNA-guided DNA endonuclease TnpB Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 46.48 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7DF80 (Residues: 1-408; Coverage: 100%)
Gene names: DR_0666, DR_0978, DR_1381, DR_1593, DR_1651, DR_1933, DR_2324, orf2, tnpB, tnpC
Sequence domains:
DNA (43-MER) Chain: C
Molecule details ›
Chain: C
Length: 43 nucleotides
Theoretical weight: 13.31 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (43-MER) Chain: D
Molecule details ›
Chain: D
Length: 43 nucleotides
Theoretical weight: 13.22 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (150-MER) Chain: B
Molecule details ›
Chain: B
Length: 150 nucleotides
Theoretical weight: 48.41 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.14Å
Relevant EMDB volumes: EMD-28656
Expression systems:
  • Escherichia coli BL21
  • Not provided