› 8exr

Electron Microscopy
3.8Å resolution

Cryo-EM structure of S. aureus BlaR1 TM and zinc metalloprotease domain

Released:
DOI: 10.2210/pdb8exr/pdb
PDB entry 8exr coloured by chain, front view.
PDB entry 8exr coloured by chain, side view.
PDB entry 8exr coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural basis of broad-spectrum β-lactam resistance in Staphylococcus aureus.
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
OpenAccess logo Nature 613 375-382 (2023)
PMID: 36599987
Related structures: EMD-28660

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-223637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein BlaR1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 602 amino acids
Theoretical weight: 71.27 KDa
Source organism: Staphylococcus aureus
Expression system: Lactobacillus delbrueckii subsp. lactis
UniProt:
  • Canonical: P18357 (Residues: 1-585; Coverage: 100%)
Gene name: blaR1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand P6L 2 x P6L
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-28660
Expression system: Lactobacillus delbrueckii subsp. lactis

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Citations

2 review citations

Molecular Determinants of β-Lactam Resistance in Methicillin-Resistant Staphylococcus aureus (MRSA): An Updated Review.
Lade et al. (2023)
1 more