› 8ey1

X-ray diffraction
3.2Å resolution

Structure of Arabidopsis fatty acid amide hydrolase mutant S305A in complex with N-(3-oxododecanoyl)-L-homoserine lactone

Released:
DOI: 10.2210/pdb8ey1/pdb
PDB entry 8ey1 coloured by chain, front view.
PDB entry 8ey1 coloured by chain, side view.
PDB entry 8ey1 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Aziz M, Wang X, Gaguancela OA, Chapman KD

Function and Biology Details

Reaction catalysed: 
Anandamide + H(2)O = arachidonic acid + ethanolamine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid amide hydrolase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 636 amino acids
Theoretical weight: 69.56 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7XJJ7 (Residues: 1-607; Coverage: 100%)
Gene names: At5g64440, FAAH, T12B11.3
Sequence domains: Amidase

Ligands and Environments

1 bound ligand:
Ligand OHN 5 x OHN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P21
Unit cell:
a: 225.544Å b: 83.75Å c: 270.943Å
α: 90° β: 109.57° γ: 90°
R-values:
R R work R free
0.26 0.259 0.29
Expression system: Escherichia coli

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