8eyc

X-ray diffraction
2.99Å resolution

Crystal structure of PTP1B D181A/Q262A/C215A phosphatase domain with TYK2 activation loop phosphopeptide

Released:
Model geometry
Fit model/data

Function and Biology Details

Reactions catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-273279 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-299; Coverage: 69%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Non-receptor tyrosine-protein kinase TYK2 Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.98 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P29597 (Residues: 1048-1062; Coverage: 1%)
Gene name: TYK2

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 44.472Å b: 88.186Å c: 97.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.217 0.254
Expression systems:
  • Escherichia coli
  • Not provided