› 8eyc

X-ray diffraction
2.99Å resolution

Crystal structure of PTP1B D181A/Q262A/C215A phosphatase domain with TYK2 activation loop phosphopeptide

Released:
DOI: 10.2210/pdb8eyc/pdb
Model geometry
Fit model/data
PDB entry 8eyc coloured by chain, front view.
PDB entry 8eyc coloured by chain, side view.
PDB entry 8eyc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure guided studies of the interaction between PTP1B and JAK.
Morris R, Keating N, Tan C, Chen H, Laktyushin A, Saiyed T, Liau NPD, Nicola NA, Tiganis T, Kershaw NJ, Babon JJ
OpenAccess logo Commun Biol 6 641 (2023)
PMID: 37316570

Function and Biology Details

Reactions catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273279 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-299; Coverage: 69%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Non-receptor tyrosine-protein kinase TYK2 Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.98 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P29597 (Residues: 1048-1062; Coverage: 1%)
Gene name: TYK2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 44.472Å b: 88.186Å c: 97.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.217 0.254
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

High-resolution double vision of the allosteric phosphatase PTP1B.
Sharma et al. (2024)
1 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO