› 8ezo

X-ray diffraction
1.9Å resolution

Lysozyme Anomalous Dataset at 220 K and 7.1 keV

Released:
DOI: 10.2210/pdb8ezo/pdb
PDB entry 8ezo coloured by chain, front view.
PDB entry 8ezo coloured by chain, side view.
PDB entry 8ezo coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Obtaining anomalous and ensemble information from protein crystals from 220 K up to physiological temperatures.
Doukov T, Herschlag D, Yabukarski F
Acta Crystallogr D Struct Biol 79 212-223 (2023)
PMID: 36876431

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

3 bound ligands:
Ligand CL 10 x CL
Ligand NA 2 x NA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P43212
Unit cell:
a: 76.909Å b: 76.909Å c: 36.961Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.191
Expression system: Gallus gallus

Quick links

Citations

2 citation in other articles

Introduction to the virtual thematic issue on room-temperature biological crystallography.
Steiner RA. (2023)
1 more