› 8f2a

Electron Microscopy
2.2Å resolution

Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385 (Cluster 5 conformation)

Released:
DOI: 10.2210/pdb8f2a/pdb
PDB entry 8f2a coloured by chain, front view.
PDB entry 8f2a coloured by chain, side view.
PDB entry 8f2a coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into selectivity of amylin and calcitonin receptor agonists.
Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM
Nat Chem Biol (2023)
PMID: 37537379
Related structures: EMD-28810

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236249 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Receptor activity-modifying protein 3 Chain: E
Molecule details ›
Chain: E
Length: 149 amino acids
Theoretical weight: 16.9 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O60896 (Residues: 24-148; Coverage: 100%)
Gene name: RAMP3
Sequence domains: Receptor activity modifying family
Islet amyloid polypeptide Chain: P
Molecule details ›
Chain: P
Length: 38 amino acids
Theoretical weight: 4 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10997 (Residues: 34-70; Coverage: 55%)
Gene name: IAPP
Sequence domains: Calcitonin / CGRP / IAPP family
Calcitonin receptor Chain: R
Molecule details ›
Chain: R
Length: 501 amino acids
Theoretical weight: 58.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P30988 (Residues: 25-474; Coverage: 100%)
Gene name: CALCR
Sequence domains:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.2Å
Relevant EMDB volumes: EMD-28810
Expression systems:
  • Trichoplusia ni
  • Not provided
  • Escherichia coli

Quick links

Citations

1 review citation

G protein-coupled receptors and obesity.
Pocai A. (2023)