› 8f2k

Electron Microscopy
2.9Å resolution

Structure of yeast F1-ATPase determined with 100 micromolar cruentaren A

Released:
DOI: 10.2210/pdb8f2k/pdb
PDB entry 8f2k coloured by chain, front view.
PDB entry 8f2k coloured by chain, side view.
PDB entry 8f2k coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
CryoEM Structure with ATP Synthase Enables Late-Stage Diversification of Cruentaren A.
Dou X, Guo H, D'Amico T, Abdallah L, Subramanian C, Patel BA, Cohen M, Rubinstein JL, Blagg BSJ
Chemistry 29 e202300262 (2023)
PMID: 36867738
Related structures: EMD-28818

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133632 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 485 amino acids
Theoretical weight: 52.38 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P07251 (Residues: 61-545; Coverage: 89%)
Gene names: ATP1, YBL0827, YBL099W
Sequence domains:
ATP synthase subunit beta, mitochondrial Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 469 amino acids
Theoretical weight: 50.38 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00830 (Residues: 41-509; Coverage: 92%)
Gene names: ATP2, J2041, YJR121W
Sequence domains:
ATP synthase subunit gamma, mitochondrial Chain: G

Ligands and Environments

3 bound ligands:
Ligand ATP 5 x ATP
Ligand MG 5 x MG
Ligand XBC 2 x XBC
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.9Å
Relevant EMDB volumes: EMD-28818

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