› 8f4r

Electron Microscopy
3.06Å resolution

Gentamicin bound aminoglycoside efflux pump AcrD

Released:
DOI: 10.2210/pdb8f4r/pdb
PDB entry 8f4r coloured by chain, front view.
PDB entry 8f4r coloured by chain, side view.
PDB entry 8f4r coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Cryo-EM Structures of AcrD Illuminate a Mechanism for Capturing Aminoglycosides from Its Central Cavity.
Zhang Z, Morgan CE, Cui M, Yu EW
OpenAccess logo mBio e0338322 (2023)
PMID: 36625574
Related structures: EMD-28857

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable aminoglycoside efflux pump Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1037 amino acids
Theoretical weight: 113.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P24177 (Residues: 1-1037; Coverage: 100%)
Gene names: JW2454, acrD, b2470, yffA
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

1 bound ligand:
Ligand LLL 1 x LLL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.06Å
Relevant EMDB volumes: EMD-28857
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Molecular insights into the determinants of substrate specificity and efflux inhibition of the RND efflux pumps AcrB and AdeB.
Wilhelm et al. (2024)
3 other articles

1 mention without citation

Cryo-EM Structures of AcrD Illuminate a Mechanism for Capturing Aminoglycosides from Its Central Cavity.
Zhang et al. (2023)