› 8f5g

X-ray diffraction
2.7Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269953 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription termination/antitermination protein NusG Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 182 amino acids
Theoretical weight: 21.28 KDa
Source organism: Thermoanaerobacter pseudethanolicus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: B0KAR9 (Residues: 1-179; Coverage: 100%)
Gene name: Teth39_1558
Sequence domains:
RNA Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 26 nucleotides
Theoretical weight: 8.29 KDa
Source organism: Bacillus velezensis FZB42
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 68.951Å b: 87.451Å c: 148.793Å
α: 90° β: 103.24° γ: 90°
R-values:
R R work R free
0.212 0.209 0.256
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

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