› 8f6b

X-ray diffraction
2.75Å resolution

Crystal structure of murine PolG2 hexamer bound to DNA

Released:
DOI: 10.2210/pdb8f6b/pdb
Model geometry
Fit model/data
PDB entry 8f6b coloured by chain, front view.
PDB entry 8f6b coloured by chain, side view.
PDB entry 8f6b coloured by chain, top view.
Source organisms:
Primary publication:
Structure-specific roles for PolG2-DNA complexes in maintenance and replication of mitochondrial DNA.
Wojtaszek JL, Hoff KE, Longley MJ, Kaur P, Andres SN, Wang H, Williams RS, Copeland WC
Nucleic Acids Res (2023)
PMID: 37592734

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270421 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase subunit gamma-2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 455 amino acids
Theoretical weight: 51.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9QZM2 (Residues: 17-459; Coverage: 97%)
Gene names: Mtpolb, Polg2
Sequence domains: Anticodon binding domain
DNA (5'-D(*CP*TP*GP*GP*TP*AP*GP*GP*CP*GP*CP*CP*TP*AP*CP*CP*AP*G)-3') Chains: G, H, I, J
Molecule details ›
Chains: G, H, I, J
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand GOL 2 x GOL
Ligand EDO 32 x EDO
Ligand MPD 2 x MPD
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P32
Unit cell:
a: 116.224Å b: 116.224Å c: 250.938Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.196 0.222
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 mention without citation

Structure-specific roles for PolG2-DNA complexes in maintenance and replication of mitochondrial DNA.
Wojtaszek et al. (2023)

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO