› 8f6t

Electron Microscopy
2.76Å resolution

Cryo-EM structure of alkane 1-monooxygenase AlkB-AlkG complex from Fontimonas thermophila

Released:
DOI: 10.2210/pdb8f6t/pdb
PDB entry 8f6t coloured by chain, front view.
PDB entry 8f6t coloured by chain, side view.
PDB entry 8f6t coloured by chain, top view.
Source organism: Fontimonas thermophila
Primary publication:
Structural basis for enzymatic terminal C-H bond functionalization of alkanes.
Chai J, Guo G, McSweeney SM, Shanklin J, Liu Q
OpenAccess logo Nat Struct Mol Biol (2023)
PMID: 36997762
Related structures: EMD-28890

Function and Biology Details

Reaction catalysed: 
Octane + 2 reduced rubredoxin + O(2) + 2 H(+) = 1-octanol + 2 oxidized rubredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rubredoxin-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 471 amino acids
Theoretical weight: 52.94 KDa
Source organism: Fontimonas thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1I2I8Z9 (Residues: 1-468; Coverage: 100%)
Gene name: SAMN04488120_10311
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FE 3 x FE
Ligand D12 1 x D12
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.76Å
Relevant EMDB volumes: EMD-28890
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Functional production and biochemical investigation of an integral membrane enzyme for olefin biosynthesis.
Murugan et al. (2024)
1 more

1 mention without citation

Structural basis for enzymatic terminal C-H bond functionalization of alkanes.
Chai et al. (2023)