› 8f7r

Electron Microscopy
3.28Å resolution

Gi bound mu-opioid receptor in complex with endomorphin

Released:
DOI: 10.2210/pdb8f7r/pdb
PDB entry 8f7r coloured by chain, front view.
PDB entry 8f7r coloured by chain, side view.
PDB entry 8f7r coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the entire human opioid receptor family.
Wang Y, Zhuang Y, DiBerto JF, Zhou XE, Schmitz GP, Yuan Q, Jain MK, Liu W, Melcher K, Jiang Y, Roth BL, Xu HE
Cell (2023)
PMID: 36638794
Related structures: EMD-28908

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-273604 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Mu-type opioid receptor Chains: M, R
Molecule details ›
Chains: M, R
Length: 403 amino acids
Theoretical weight: 45.48 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P35372 (Residues: 2-388; Coverage: 97%)
  • Best match: P35372-4 (Residues: 2-388)
Gene names: MOR1, OPRM1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
endomorphin Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 5 amino acids
Theoretical weight: 610 Da
Source organism: Homo sapiens
Expression system: Not provided
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, F
Molecule details ›
Chains: A, F
Length: 354 amino acids
Theoretical weight: 40.45 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 353 amino acids
Theoretical weight: 39.02 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 68 amino acids
Theoretical weight: 7.56 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-68; Coverage: 96%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 248 amino acids
Theoretical weight: 26.41 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CLR 10 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.28Å
Relevant EMDB volumes: EMD-28908
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Escherichia coli

Quick links

Citations

13 review citations

Molecular and Epigenetic Aspects of Opioid Receptors in Drug Addiction and Pain Management in Sport.
Mazzeo et al. (2023)
12 more

4 mentions without citation

Structure-Activity Relationship Target Prediction Studies of Clindamycin Derivatives with Broad-Spectrum Bacteriostatic Antibacterial Properties.
Jia et al. (2023)
3 more