› 8f8f

X-ray diffraction
2Å resolution

The structure of Rv2173 from M. tuberculosis (APO form)

Released:
DOI: 10.2210/pdb8f8f/pdb
PDB entry 8f8f coloured by chain, front view.
PDB entry 8f8f coloured by chain, side view.
PDB entry 8f8f coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Johnston JM, Allison TM, Titterington J

Function and Biology Details

Reactions catalysed: 
Prenyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(2E,6E)-farnesyl diphosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 42.06 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53507 (Residues: 1-352; Coverage: 100%)
Gene names: Rv2173, idsA2
Sequence domains: Polyprenyl synthetase

Ligands and Environments

2 bound ligands:
Ligand GOL 9 x GOL
Ligand MG 1 x MG
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21212
Unit cell:
a: 81.797Å b: 190.713Å c: 66.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.238
Expression system: Escherichia coli BL21(DE3)

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