› 8fbz

X-ray diffraction
1.59Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 477 amino acids
Theoretical weight: 52.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48637 (Residues: 1-474; Coverage: 100%)
Gene name: GSS
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Unit cell:
a: 80.834Å b: 94.051Å c: 125.122Å
α: 90° β: 92.522° γ: 90°
R-values:
R R work R free
0.161 0.16 0.189
Expression system: Escherichia coli BL21(DE3)

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