› 8fcx

Electron Microscopy
3.04Å resolution

Cryo-EM structure of TnsC oligomer in type I-B CAST system

Released:
DOI: 10.2210/pdb8fcx/pdb
PDB entry 8fcx coloured by chain, front view.
PDB entry 8fcx coloured by chain, side view.
PDB entry 8fcx coloured by chain, top view.
Source organism: Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
Primary publication:
Molecular mechanism for Tn7-like transposon recruitment by a type I-B CRISPR effector.
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023)
PMID: 37557170
Related structures: EMD-28998

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224618 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORC1/DEAH AAA+ ATPase domain-containing protein Chains: R, S, T, U, V
Molecule details ›
Chains: R, S, T, U, V
Length: 383 amino acids
Theoretical weight: 43.52 KDa
Source organism: Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A235IFM2 (Residues: 1-383; Coverage: 100%)
Gene name: CDG76_08990
Sequence domains: AAA domain

Ligands and Environments

2 bound ligands:
Ligand ATP 5 x ATP
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.04Å
Relevant EMDB volumes: EMD-28998
Expression system: Escherichia coli

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Citations

1 review citation

DNA on the move: mechanisms, functions and applications of transposable elements.
Schmitz et al. (2024)