› 8fd3

Electron Microscopy
3.12Å resolution

Cryo-EM structure of Cascade-PAM complex in type I-B CAST system

Released:
DOI: 10.2210/pdb8fd3/pdb
PDB entry 8fd3 coloured by chain, front view.
PDB entry 8fd3 coloured by chain, side view.
PDB entry 8fd3 coloured by chain, top view.
Source organism: Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
Primary publication:
Molecular mechanism for Tn7-like transposon recruitment by a type I-B CRISPR effector.
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023)
PMID: 37557170
Related structures: EMD-29001

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-224623 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
Type I-MYXAN CRISPR-associated protein Cas5/Cmx5/DevS Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 24.85 KDa
Source organism: Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A235IG00 (Residues: 1-212; Coverage: 100%)
Gene names: CDG76_09075, cas5
Type I-MYXAN CRISPR-associated protein Cas6/Cmx6 Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 24.95 KDa
Source organism: Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A235IH92 (Residues: 1-220; Coverage: 100%)
Gene names: CDG76_09090, cas6
Sequence domains: Cas6 Crispr
CRISPR-associated protein Chains: C, D, E, F, G, H
Molecule details ›
Chains: C, D, E, F, G, H
Length: 323 amino acids
Theoretical weight: 37.3 KDa
Source organism: Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A235IG15 (Residues: 1-323; Coverage: 100%)
Gene name: CDG76_09080
Type I-MYXAN CRISPR-associated Cas8a1/Cmx1 Chain: I
Molecule details ›
Chain: I
Length: 534 amino acids
Theoretical weight: 63.47 KDa
Source organism: Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A235IGR9 (Residues: 2-525; Coverage: 100%)
Gene name: CDG76_09085
Type I-MYXAN CRISPR-associated Cas8a1/Cmx1 Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 138 amino acids
Theoretical weight: 16.31 KDa
Source organism: Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A235IGR9 (Residues: 388-525; Coverage: 26%)
Gene name: CDG76_09085
Target DNA strand Chain: N
Molecule details ›
Chain: N
Length: 10 nucleotides
Theoretical weight: 3 KDa
Non-target DNA strand Chain: O
Molecule details ›
Chain: O
Length: 45 nucleotides
Theoretical weight: 13.97 KDa
RNA Chain: M
Molecule details ›
Chain: M
Length: 71 nucleotides
Theoretical weight: 22.88 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.12Å
Relevant EMDB volumes: EMD-29001
Expression system: Escherichia coli

Quick links

Citations

1 review citation

DNA on the move: mechanisms, functions and applications of transposable elements.
Schmitz et al. (2024)