› 8fd8

Electron Microscopy
3.3Å resolution

human 15-PGDH with NADH bound

Released:
DOI: 10.2210/pdb8fd8/pdb
PDB entry 8fd8 coloured by chain, front view.
PDB entry 8fd8 coloured by chain, side view.
PDB entry 8fd8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Small molecule inhibitors of 15-PGDH exploit a physiologic induced-fit closing system.
Huang W, Li H, Kiselar J, Fink SP, Regmi S, Day A, Yuan Y, Chance M, Ready JM, Markowitz SD, Taylor DJ
OpenAccess logo Nat Commun 14 784 (2023)
PMID: 36774348
Related structures: EMD-29005

Function and Biology Details

Reactions catalysed: 
(5Z,13E,15S)-11-alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD(+) = (5Z,13E)-11-alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH
(15S)-15-hydroxy-5,8,11-cis-13-trans-icosatetraenoate + NAD(P)(+) = 15-oxo-5,8,11-cis-13-trans-icosatetraenoate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
15-hydroxyprostaglandin dehydrogenase [NAD(+)] Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 27.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15428 (Residues: 1-256; Coverage: 96%)
Gene names: HPGD, PGDH1, SDR36C1
Sequence domains: short chain dehydrogenase

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-29005
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Inhibition of Prostaglandin-Degrading Enzyme 15-PGDH Mitigates Acute Murine Lung Allograft Rejection.
Cui et al. (2023)