› 8feq

X-ray diffraction
1.5Å resolution

16mer self-complementary duplex RNA with two separated s(2)U:s(2)U pairs

Released:
DOI: 10.2210/pdb8feq/pdb
PDB entry 8feq coloured by chain, front view.
PDB entry 8feq coloured by chain, side view.
PDB entry 8feq coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Unusual Base Pair between Two 2-Thiouridines and Its Implication for Nonenzymatic RNA Copying.
Ding D, Fang Z, Kim SC, O'Flaherty DK, Jia X, Stone TB, Zhou L, Szostak JW
J Am Chem Soc (2024)
PMID: 38293747

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239181 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA 16mer with two separated s(2)U Chain: AAA
Molecule details ›
Chain: AAA
Length: 16 nucleotides
Theoretical weight: 5.09 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand SUR 2 x SUR

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 2.0.1
Spacegroup: R32
Unit cell:
a: 41.261Å b: 41.261Å c: 124.239Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.21
Expression system: Not provided

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