› 8ff6

X-ray diffraction
2.49Å resolution

Cytosolic ascorbate peroxidase mutant from Panicum virgatum

Released:
DOI: 10.2210/pdb8ff6/pdb
PDB entry 8ff6 coloured by chain, front view.
PDB entry 8ff6 coloured by chain, side view.
PDB entry 8ff6 coloured by chain, top view.
Source organism: Panicum virgatum
Primary publication:
Activity of Cytosolic Ascorbate Peroxidase (APX) from Panicum virgatum against Ascorbate and Phenylpropanoids.
Zhang B, Lewis JA, Kovacs F, Sattler SE, Sarath G, Kang C
OpenAccess logo Int J Mol Sci 24 (2023)
PMID: 36675291

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-ascorbate + H(2)O(2) + 2 H(+) = 2 monodehydroascorbate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-ascorbate peroxidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 250 amino acids
Theoretical weight: 27.3 KDa
Source organism: Panicum virgatum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A8T0NWI5 (Residues: 1-250; Coverage: 100%)
Gene name: PVAP13_9KG480900
Sequence domains: Peroxidase

Ligands and Environments


Cofactor: Ligand HEM 6 x HEM
1 bound ligand:
Ligand NA 6 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P1
Unit cell:
a: 78.558Å b: 79.984Å c: 80.147Å
α: 104.33° β: 111.17° γ: 101.78°
R-values:
R R work R free
0.19 0.188 0.262
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Transcriptomic Complexity of Culm Growth and Development in Different Types of Moso Bamboo.
Li et al. (2023)
1 other article

1 mention without citation

Activity of Cytosolic Ascorbate Peroxidase (APX) from Panicum virgatum against Ascorbate and Phenylpropanoids.
Zhang et al. (2023)