8fh3

Electron Microscopy
4.3Å resolution

Human IFT-A complex structures provide molecular insights into ciliary transport

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-130939 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
WD repeat-containing protein 35 Chain: A
Molecule details ›
Chain: A
Length: 1181 amino acids
Theoretical weight: 133.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9P2L0 (Residues: 1-1181; Coverage: 100%)
Gene names: IFT121, KIAA1336, WDR35
Sequence domains:
Intraflagellar transport protein 122 homolog Chain: B
Molecule details ›
Chain: B
Length: 1241 amino acids
Theoretical weight: 141.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9HBG6 (Residues: 1-1241; Coverage: 100%)
Gene names: IFT122, SPG, WDR10, WDR140
Sequence domains: WD domain, G-beta repeat
WD repeat-containing protein 19 Chain: C
Molecule details ›
Chain: C
Length: 1342 amino acids
Theoretical weight: 151.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NEZ3 (Residues: 1-1342; Coverage: 100%)
Gene names: IFT144, KIAA1638, WDR19
Sequence domains:
Intraflagellar transport protein 140 homolog Chain: E
Molecule details ›
Chain: E
Length: 1462 amino acids
Theoretical weight: 165.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96RY7 (Residues: 1-1462; Coverage: 100%)
Gene names: IFT140, KIAA0590, WDTC2
Tubby-related protein 3 Chain: I
Molecule details ›
Chain: I
Length: 442 amino acids
Theoretical weight: 49.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75386 (Residues: 1-442; Coverage: 100%)
Gene names: TUBL3, TULP3
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-29078
Expression system: Homo sapiens