› 8fhq

Electron Microscopy
2.81Å resolution

Cryo-EM structure of human NCC (class 3-2)

Released:
DOI: 10.2210/pdb8fhq/pdb
PDB entry 8fhq coloured by chain, front view.
PDB entry 8fhq coloured by chain, side view.
PDB entry 8fhq coloured by chain, top view.
Source organisms:
Primary publication:
Structure and thiazide inhibition mechanism of the human Na-Cl cotransporter.
Fan M, Zhang J, Lee CL, Zhang J, Feng L
Nature (2023)
PMID: 36792826
Related structures: EMD-29099

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101344 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute carrier family 12 member 2; Solute carrier family 12 member 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1092 amino acids
Theoretical weight: 120.37 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: A0A0G2KTI4 (Residues: 1-202, 335-335; Coverage: 18%)
  • Canonical: P55017 (Residues: 132-263, 265-1021; Coverage: 87%)
Gene names: NCC, SLC12A3, TSC, nkcc1, slc12a2
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand XZF 2 x XZF
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.81Å
Relevant EMDB volumes: EMD-29099
Expression system: Homo sapiens

Quick links

Citations

2 review citations

The role of SLC12A family of cation-chloride cotransporters and drug discovery methodologies.
Zhang et al. (2023)
1 more

1 mention without citation

Structure and thiazide inhibition mechanism of the human Na-Cl cotransporter.
Fan et al. (2023)