› 8fhw

Electron Microscopy
3.2Å resolution

Cryo-EM structure of Cryptococcus neoformans trehalose-6-phosphate synthase homotetramer in complex with uridine diphosphate and glucose-6-phosphate

Released:
DOI: 10.2210/pdb8fhw/pdb
PDB entry 8fhw coloured by chain, front view.
PDB entry 8fhw coloured by chain, side view.
PDB entry 8fhw coloured by chain, top view.
Source organism: Cryptococcus neoformans var. grubii H99
Primary publication:
Structures of trehalose-6-phosphate synthase, Tps1, from the fungal pathogen Cryptococcus neoformans: a target for novel antifungals.
Washington EJ, Zhou Y, Hsu AL, Petrovich M, Borgnia MJ, Bartesaghi A, Brennan RG
OpenAccess logo bioRxiv (2023)
PMID: 36993618
Related structures: EMD-29172

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236907 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha,alpha-trehalose-phosphate synthase (UDP-forming) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 693 amino acids
Theoretical weight: 76.82 KDa
Source organism: Cryptococcus neoformans var. grubii H99
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J9VJZ1 (Residues: 1-669; Coverage: 100%)
Gene name: CNAG_05292
Sequence domains: Glycosyltransferase family 20

Ligands and Environments

2 bound ligands:
Ligand UDP 4 x UDP
Ligand G6P 4 x G6P
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-29172
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Structures of trehalose-6-phosphate synthase, Tps1, from the fungal pathogen Cryptococcus neoformans : a target for novel antifungals.
Washington et al. (2024)