8fks

Electron Microscopy
2.88Å resolution

Human nucleolar pre-60S ribosomal subunit (State B2)

Released:
Source organism: Homo sapiens
Primary publication:
Principles of human pre-60S biogenesis.
Science 381 eadh3892 (2023)
PMID: 37410842
Related structures: EMD-29255

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 47-mer (preferred)
PDBe Complex ID:
PDB-CPX-271471 (preferred)
Entry contents:
44 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (47 distinct):
Large ribosomal subunit protein uL11 Chain: BA
Molecule details ›
Chain: BA
Length: 165 amino acids
Theoretical weight: 17.85 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P30050 (Residues: 1-165; Coverage: 100%)
Gene name: RPL12
Sequence domains:
Large ribosomal subunit protein eL13 Chain: L6
Molecule details ›
Chain: L6
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P26373 (Residues: 1-211; Coverage: 100%)
Gene names: BBC1, OK/SW-cl.46, RPL13
Sequence domains: Ribosomal protein L13e
Large ribosomal subunit protein uL13 Chain: L7
Large ribosomal subunit protein eL14 Chain: L8
Large ribosomal subunit protein eL15 Chain: L9
Large ribosomal subunit protein uL22 Chain: LA
Large ribosomal subunit protein eL18 Chain: LB
Large ribosomal subunit protein eL20 Chain: LC
Large ribosomal subunit protein eL21 Chain: LE
Large ribosomal subunit protein uL14 Chain: LG
Large ribosomal subunit protein uL23 Chain: LH
Large ribosomal subunit protein uL24 Chain: LI
Large ribosomal subunit protein uL15 Chain: LK
Large ribosomal subunit protein eL28 Chain: LL
Large ribosomal subunit protein uL3 Chain: LN
Large ribosomal subunit protein eL32 Chain: LQ
Large ribosomal subunit protein uL29 Chain: LS
Large ribosomal subunit protein eL33 Chain: LT
Large ribosomal subunit protein eL36 Chain: LU
Large ribosomal subunit protein eL37 Chain: LW
Surfeit locus protein 6 Chain: NE
Ribosome biogenesis protein NSA2 homolog Chain: NF
RRP15-like protein Chain: NG
Suppressor of SWI4 1 homolog Chain: NN
Large ribosomal subunit protein uL4 Chain: SA
Large ribosomal subunit protein eL6 Chain: SC
Large ribosomal subunit protein uL30 Chain: SD
Large ribosomal subunit protein eL8 Chain: SE
Large ribosomal subunit protein uL6 Chain: SG
MKI67 FHA domain-interacting nucleolar phosphoprotein Chain: SH
Ribosomal protein uL30-like Chain: SI
pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 Chain: SJ
Eukaryotic translation initiation factor 6 Chain: SK
Ribosomal L1 domain-containing protein 1 Chain: SL
Pescadillo homolog Chain: SM
Probable rRNA-processing protein EBP2 Chain: SN
Ribosome biogenesis protein BRX1 homolog Chain: SO
mRNA turnover protein 4 homolog Chain: SQ
GTP-binding protein 4 Chain: SR
Ribosome biogenesis protein BOP1 Chain: SS
Ribosome biogenesis regulatory protein homolog Chain: ST
Probable ribosome biogenesis protein RLP24 Chain: SV
ATP-dependent RNA helicase DDX18 Chain: SW
Nucleolar protein 16 Chain: SZ
5.8S ribosomal RNA Chain: L1
ITS2 rRNA Chain: L2
28S ribosomal RNA Chain: L3

Ligands and Environments

2 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-29255