› 8fl5

X-ray diffraction
1.8Å resolution

Crystal Structure of Enterovirus 68 3C Protease inactive mutant C147A at 1.8 Angstroms.

Released:
DOI: 10.2210/pdb8fl5/pdb
PDB entry 8fl5 coloured by chain, front view.
PDB entry 8fl5 coloured by chain, side view.
PDB entry 8fl5 coloured by chain, top view.
Source organism: enterovirus D68
Entry authors: Azzolino VN, Shaqra AM, Schiffer CA

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3C Protease Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.2 KDa
Source organism: enterovirus D68
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A097BW12 (Residues: 1549-1731; Coverage: 8%)
Sequence domains: 3C cysteine protease (picornain 3C)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 39.166Å b: 102.109Å c: 41.946Å
α: 90° β: 109.88° γ: 90°
R-values:
R R work R free
0.18 0.176 0.239
Expression system: Escherichia coli

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