› 8fls

Electron Microscopy
3.09Å resolution

Human PTH1R in complex with Abaloparatide and Gs

Released:
DOI: 10.2210/pdb8fls/pdb
PDB entry 8fls coloured by chain, front view.
PDB entry 8fls coloured by chain, side view.
PDB entry 8fls coloured by chain, top view.
Source organisms:
Primary publication:
Molecular insights into peptide agonist engagement with the PTH receptor.
Cary BP, Gerrard EJ, Belousoff MJ, Fletcher MM, Jiang Y, Russell IC, Piper SJ, Wootten D, Sexton PM
Structure (2023)
PMID: 37148874
Related structures: EMD-29285

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-273953 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.41 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 5-62; Coverage: 82%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 128 amino acids
Theoretical weight: 13.89 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Abaloparatide Chain: P
Molecule details ›
Chain: P
Length: 34 amino acids
Theoretical weight: 3.97 KDa
Source organism: synthetic construct
Expression system: Not provided
Parathyroid hormone/parathyroid hormone-related peptide receptor Chain: R
Molecule details ›
Chain: R
Length: 616 amino acids
Theoretical weight: 69.51 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q03431 (Residues: 28-593; Coverage: 100%)
Gene names: PTH1R, PTHR, PTHR1
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand AIB 1 x AIB

Experiments and Validation Details

Entry percentile scores
Resolution: 3.09Å
Relevant EMDB volumes: EMD-29285
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided

Quick links

Citations

3 citation in other articles

Molecular recognition of two endogenous hormones by the human parathyroid hormone receptor-1.
Zhao et al. (2023)
2 more