› 8fm1

X-ray diffraction
3.16Å resolution

Structure of CBASS Cap5 from Pseudomonas syringae in the absence of a ligand (apo form dimer)

Released:
DOI: 10.2210/pdb8fm1/pdb
PDB entry 8fm1 coloured by chain, front view.
PDB entry 8fm1 coloured by chain, side view.
PDB entry 8fm1 coloured by chain, top view.
Source organism: Pseudomonas syringae
Primary publication:
Activation of CBASS Cap5 endonuclease immune effector by cyclic nucleotides.
Rechkoblit O, Sciaky D, Kreitler DF, Buku A, Kottur J, Aggarwal AK
Nat Struct Mol Biol (2024)
PMID: 38321146

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HNH endonuclease Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 388 amino acids
Theoretical weight: 42.77 KDa
Source organism: Pseudomonas syringae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2P0QGK5 (Residues: 10-397; Coverage: 98%)
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P41212
Unit cell:
a: 159.428Å b: 159.428Å c: 433.875Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.24 0.294
Expression system: Escherichia coli

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