› 8fmf

X-ray diffraction
2.1Å resolution

Structure of CBASS Cap5 from Pseudomonas syringae as an activated tetramer with the cyclic dinucleotide 3'2'-c-diAMP ligand (1 tetramer in the AU)

Released:
DOI: 10.2210/pdb8fmf/pdb
PDB entry 8fmf coloured by chain, front view.
PDB entry 8fmf coloured by chain, side view.
PDB entry 8fmf coloured by chain, top view.
Source organism: Pseudomonas syringae
Primary publication:
Activation of CBASS Cap5 endonuclease immune effector by cyclic nucleotides.
Rechkoblit O, Sciaky D, Kreitler DF, Buku A, Kottur J, Aggarwal AK
Nat Struct Mol Biol (2024)
PMID: 38321146

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HNH endonuclease Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 42.77 KDa
Source organism: Pseudomonas syringae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2P0QGK5 (Residues: 10-397; Coverage: 98%)
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand Y4F 4 x Y4F
Ligand GOL 6 x GOL
Ligand RWB 1 x RWB
Ligand ZN 4 x ZN
Ligand MG 7 x MG
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P3221
Unit cell:
a: 83.536Å b: 83.536Å c: 401.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.237
Expression system: Escherichia coli

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