› 8fn0

Electron Microscopy
2.89Å resolution

CryoEM structure of Go-coupled NTSR1 with a biased allosteric modulator

Released:
DOI: 10.2210/pdb8fn0/pdb
PDB entry 8fn0 coloured by chain, front view.
PDB entry 8fn0 coloured by chain, side view.
PDB entry 8fn0 coloured by chain, top view.
Source organisms:
Primary publication:
Neurotensin Receptor Allosterism Revealed in Complex with a Biased Allosteric Modulator.
Krumm BE, DiBerto JF, Olsen RHJ, Kang HJ, Slocum ST, Zhang S, Strachan RT, Huang XP, Slosky LM, Pinkerton AB, Barak LS, Caron MG, Kenakin T, Fay JF, Roth BL
Biochemistry (2023)
PMID: 36917754
Related structures: EMD-29302

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-210781 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Neurotensin receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 45.94 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P20789 (Residues: 43-424; Coverage: 90%)
Gene names: Ntsr, Ntsr1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Neurotensin Chain: F
Molecule details ›
Chain: F
Length: 6 amino acids
Theoretical weight: 819 Da
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P20068 (Residues: 157-162; Coverage: 4%)
Gene name: Nts
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
MiniGo Chain: B
Molecule details ›
Chain: B
Length: 228 amino acids
Theoretical weight: 25.45 KDa
Source organism: Escherichia coli
Expression system: Spodoptera frugiperda
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 267 amino acids
Theoretical weight: 28.67 KDa
Source organism: Lama glama
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand SRW 1 x SRW
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.89Å
Relevant EMDB volumes: EMD-29302
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

2 review citations

Allosteric modulation of G protein-coupled receptor signaling.
Shen et al. (2023)
1 more

1 mention without citation

Structure, function and drug discovery of GPCR signaling.
Cheng et al. (2023)