› 8fpw

X-ray diffraction
1.4Å resolution

Crystal structure of tumor related RhoA mutant A161V in complex with GDP

Released:
DOI: 10.2210/pdb8fpw/pdb
PDB entry 8fpw coloured by chain, front view.
PDB entry 8fpw coloured by chain, side view.
PDB entry 8fpw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tumor-derived RHOA mutants interact with effectors in the GDP-bound state.
Lin Y, Ramelot TA, Senyuz S, Gursoy A, Jang H, Nussinov R, Keskin O, Zheng Y
Nat Commun 15 7176 (2024)
PMID: 39169042

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transforming protein RhoA Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.61 KDa
Source organism: Homo sapiens
Expression system: Bacteria
UniProt:
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
Ligand DIO 2 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 32.01Å b: 86.844Å c: 34.337Å
α: 90° β: 93.75° γ: 90°
R-values:
R R work R free
0.18 0.178 0.208
Expression system: Bacteria

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