› 8fqc

Electron Microscopy
3.2Å resolution

Structure of baseplate with receptor binding complex of Agrobacterium phage Milano

Released:
DOI: 10.2210/pdb8fqc/pdb
PDB entry 8fqc coloured by chain, front view.
PDB entry 8fqc coloured by chain, side view.
PDB entry 8fqc coloured by chain, top view.
Source organism: Agrobacterium phage Milano
Primary publication:
An extensive disulfide bond network prevents tail contraction in Agrobacterium tumefaciens phage Milano.
Sonani RR, Palmer LK, Esteves NC, Horton AA, Sebastian AL, Kelly RJ, Wang F, Kreutzberger MAB, Russell WK, Leiman PG, Scharf BE, Egelman EH
OpenAccess logo Nat Commun 15 756 (2024)
PMID: 38272938
Related structures: EMD-29383

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 114-mer (preferred)
PDBe Complex ID:
PDB-CPX-239286 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Baseplate hub protein Chain: C1
Molecule details ›
Chain: C1
Length: 457 amino acids
Theoretical weight: 49.7 KDa
Source organism: Agrobacterium phage Milano
UniProt:
  • Canonical: A0A482MFS1 (Residues: 1-457; Coverage: 100%)
Gene name: Milano_026
Virion-associated protein Chains: E1, a1, f1, g1
Molecule details ›
Chains: E1, a1, f1, g1
Length: 136 amino acids
Theoretical weight: 14.67 KDa
Source organism: Agrobacterium phage Milano
UniProt:
  • Canonical: A0A482MHE7 (Residues: 1-136; Coverage: 100%)
Gene name: Milano_021
Baseplate protein J-like domain-containing protein Chains: F1, G1, h1, i1
Molecule details ›
Chains: F1, G1, h1, i1
Length: 396 amino acids
Theoretical weight: 43.32 KDa
Source organism: Agrobacterium phage Milano
UniProt:
  • Canonical: A0A482MFU4 (Residues: 1-396; Coverage: 100%)
Gene name: Milano_029
Sequence domains: Baseplate J-like protein
Baseplate wedge 1, gp28 Chains: H1, j1
Molecule details ›
Chains: H1, j1
Length: 178 amino acids
Theoretical weight: 19.32 KDa
Source organism: Agrobacterium phage Milano
Tail sheath protein Chains: I1, K1, k1, m1
Molecule details ›
Chains: I1, K1, k1, m1
Length: 503 amino acids
Theoretical weight: 53.9 KDa
Source organism: Agrobacterium phage Milano
UniProt:
  • Canonical: A0A482MFS8 (Residues: 1-503; Coverage: 100%)
Gene name: Milano_020
Virion-associated protein Chains: J1, l1
Molecule details ›
Chains: J1, l1
Length: 286 amino acids
Theoretical weight: 31.41 KDa
Source organism: Agrobacterium phage Milano
UniProt:
  • Canonical: A0A482MFT7 (Residues: 1-286; Coverage: 100%)
Gene name: Milano_030
Tail fiber protein Chains: P1, Q1, R1, r1, s1, t1
Molecule details ›
Chains: P1, Q1, R1, r1, s1, t1
Length: 587 amino acids
Theoretical weight: 61.65 KDa
Source organism: Agrobacterium phage Milano
UniProt:
  • Canonical: A0A482MGV0 (Residues: 1-587; Coverage: 100%)
Gene name: Milano_124
Virion-associated protein Chains: S1, T1, V1, W1, X1, Y1, u1, v1, w1, x1, y1, z1
Molecule details ›
Chains: S1, T1, V1, W1, X1, Y1, u1, v1, w1, x1, y1, z1
Length: 300 amino acids
Theoretical weight: 31.67 KDa
Source organism: Agrobacterium phage Milano
UniProt:
  • Canonical: A0A482MHF3 (Residues: 1-300; Coverage: 100%)
Gene name: Milano_031
DNA circulation N-terminal domain-containing protein Chains: U1, e1
Molecule details ›
Chains: U1, e1
Length: 398 amino acids
Theoretical weight: 43.29 KDa
Source organism: Agrobacterium phage Milano
UniProt:
  • Canonical: A0A482MFQ9 (Residues: 1-398; Coverage: 100%)
Gene name: Milano_025
Sequence domains: DNA circularisation protein N-terminus
Baseplate protein Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 20.72 KDa
Source organism: Agrobacterium phage Milano
UniProt:
  • Canonical: A0A482MFZ0 (Residues: 1-188; Coverage: 100%)
Gene name: Milano_027

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-29383

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