8fs6

Electron Microscopy
2.9Å resolution

Structure of S. cerevisiae Rad24-RFC loading the 9-1-1 clamp onto a 10-nt gapped DNA in step 4 (partially closed 9-1-1 and stably bound chamber DNA)

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-234957 (preferred)
Entry contents:
8 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (11 distinct):
Checkpoint protein RAD24 Chain: A
Molecule details ›
Chain: A
Length: 545 amino acids
Theoretical weight: 62.79 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32641 (Residues: 1-545; Coverage: 83%)
Gene names: RAD24, SYGP-ORF60, YER173W
Sequence domains: Rad17 P-loop domain
Replication factor C subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 323 amino acids
Theoretical weight: 36.2 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40339 (Residues: 1-323; Coverage: 100%)
Gene names: O0923, RFC4, YOL094C
Sequence domains:
Replication factor C subunit 3 Chain: C
Replication factor C subunit 2 Chain: D
Replication factor C subunit 5 Chain: E
DNA damage checkpoint control protein MEC3 Chain: F
DNA damage checkpoint control protein RAD17 Chain: G
DNA damage checkpoint protein 1 Chain: H
Template strand Chain: I
Primer strand 1 Chain: J
Primer strand 2 Chain: K

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.9Å
Relevant EMDB volumes: EMD-29415
Expression system: Saccharomyces cerevisiae