› 8fuc

X-ray diffraction
2.1Å resolution

Crystal structure of mouse Importin alpha in complex with Hendra virus matrix protein minor site NLS2

Released:
DOI: 10.2210/pdb8fuc/pdb
PDB entry 8fuc coloured by chain, front view.
PDB entry 8fuc coloured by chain, side view.
PDB entry 8fuc coloured by chain, top view.
Source organisms:
Primary publication:
Henipavirus Matrix Protein Employs a Non-Classical Nuclear Localization Signal Binding Mechanism.
Donnelly CM, Vogel OA, Edwards MR, Taylor PE, Roby JA, Forwood JK, Basler CF
OpenAccess logo Viruses 15 (2023)
PMID: 37376602

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-131618 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Matrix protein Chain: A
Molecule details ›
Chain: A
Length: 18 amino acids
Theoretical weight: 2.24 KDa
Source organism: Hendra virus horse/Australia/Hendra/1994
Expression system: Not provided
UniProt:
  • Canonical: O89341 (Residues: 243-260; Coverage: 5%)
Gene name: M
Importin subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 510 amino acids
Theoretical weight: 55.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Contaminant peptide KKLARE Chain: F
Molecule details ›
Chain: F
Length: 6 amino acids
Theoretical weight: 747 Da
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 78.55Å b: 90.18Å c: 98.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.217
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Henipavirus Matrix Protein Employs a Non-Classical Nuclear Localization Signal Binding Mechanism.
Donnelly et al. (2023)