› 8fxt

X-ray diffraction
1.53Å resolution

Escherichia coli periplasmic Glucose-Binding Protein glucose complex: Acrylodan conjugate attached at W183C

Released:
DOI: 10.2210/pdb8fxt/pdb
PDB entry 8fxt coloured by chain, front view.
PDB entry 8fxt coloured by chain, side view.
PDB entry 8fxt coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Chromophore carbonyl twisting in fluorescent biosensors encodes direct readout of protein conformations with multicolor switching.
Allert MJ, Kumar S, Wang Y, Beese LS, Hellinga HW
OpenAccess logo Commun Chem 6 168 (2023)
PMID: 37598249

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactose/methyl-galactoside binding periplasmic protein MglB Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 32.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE5 (Residues: 26-329; Coverage: 98%)
Gene names: JW2137, b2150, mglB
Sequence domains: Periplasmic binding protein domain

Ligands and Environments

4 bound ligands:
Ligand YDM 1 x YDM
Ligand GLC 1 x GLC
Ligand BGC 5 x BGC
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 119.587Å b: 36.533Å c: 79.905Å
α: 90° β: 124.13° γ: 90°
R-values:
R R work R free
0.157 0.156 0.183
Expression system: Escherichia coli

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Citations

1 mention without citation

Chromophore carbonyl twisting in fluorescent biosensors encodes direct readout of protein conformations with multicolor switching.
Allert et al. (2023)