8fyd

Electron Microscopy
3.9Å resolution

Cryo-EM structure of Cas1:Cas2-DEDDh:half-site integration complex bent CRISPR repeat conformation

Released:
Source organism: Megasphaera
Related structures: EMD-29565

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-215090 (preferred)
Entry contents:
2 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (6 distinct):
Cas2-DEDDh Chains: A, D
Molecule details ›
Chains: A, D
Length: 289 amino acids
Theoretical weight: 32.63 KDa
Source organism: Megasphaera
Expression system: Escherichia coli BL21(DE3)
Cas1 Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 316 amino acids
Theoretical weight: 35.02 KDa
Source organism: Megasphaera
Expression system: Escherichia coli BL21(DE3)
DNA (49-MER) Chain: J
Molecule details ›
Chain: J
Length: 78 nucleotides
Theoretical weight: 23.95 KDa
DNA (64-MER) Chain: G
Molecule details ›
Chain: G
Length: 64 nucleotides
Theoretical weight: 19.72 KDa
DNA (30-MER) Chain: H
Molecule details ›
Chain: H
Length: 33 nucleotides
Theoretical weight: 10.11 KDa
DNA (13-MER) Chain: I
Molecule details ›
Chain: I
Length: 44 nucleotides
Theoretical weight: 13.66 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-29565
Expression system: Escherichia coli BL21(DE3)