› 8fzt

Electron Microscopy
3.03Å resolution

SpCas9 with dual-guide RNA and target DNA

Released:
DOI: 10.2210/pdb8fzt/pdb
PDB entry 8fzt coloured by chain, front view.
PDB entry 8fzt coloured by chain, side view.
PDB entry 8fzt coloured by chain, top view.
Source organisms:
Entry authors: Korolev S, Gagnon K
Related structures: EMD-29639

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216618 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: E
Molecule details ›
Chain: E
Length: 1384 amino acids
Theoretical weight: 160.77 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
E2_TS Chain: C
Molecule details ›
Chain: C
Length: 40 nucleotides
Theoretical weight: 12.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
E2-NTS Chain: D
Molecule details ›
Chain: D
Length: 40 nucleotides
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: Not provided
CrE2 Chain: A
Molecule details ›
Chain: A
Length: 42 nucleotides
Theoretical weight: 13.37 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
tracrRNA Chain: B
Molecule details ›
Chain: B
Length: 70 nucleotides
Theoretical weight: 22.57 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.03Å
Relevant EMDB volumes: EMD-29639
Expression systems:
  • Escherichia coli
  • Not provided

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