› 8fzv

X-ray diffraction
3.29Å resolution

The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Ala-Ala linker variant, expressed with SUMO tag

Released:
DOI: 10.2210/pdb8fzv/pdb
Model geometry
Fit model/data
PDB entry 8fzv coloured by chain, front view.
PDB entry 8fzv coloured by chain, side view.
PDB entry 8fzv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Increasing the bulk of the 1TEL-target linker and retaining the 10×His tag in a 1TEL-CMG2-vWa construct improves crystal order and diffraction limits.
Gajjar PL, Pedroza Romo MJ, Litchfield CM, Callahan M, Redd N, Nawarathnage S, Soleimani S, Averett J, Wilson E, Lewis A, Stewart C, Tseng YJ, Doukov T, Lebedev A, Moody JD
OpenAccess logo Acta Crystallogr D Struct Biol (2023)
PMID: 37747038

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthrax toxin receptor 2; Transcription factor ETV6 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 257 amino acids
Theoretical weight: 28.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41212 (Residues: 47-111; Coverage: 14%)
  • Canonical: P58335 (Residues: 40-217; Coverage: 39%)
Gene names: ANTXR2, CMG2, ETV6, TEL, TEL1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand UNX 90 x UNX
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P65
Unit cell:
a: 162.557Å b: 162.557Å c: 56.877Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.284 0.282 0.314
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Increasing the bulk of the 1TEL-target linker and retaining the 10×His tag in a 1TEL-CMG2-vWa construct improves crystal order and diffraction limits.
Gajjar et al. (2023)