› 8fzz

X-ray diffraction
2.68Å resolution

Phocaeicola vulgatus type VI secretion system Ntox15 effector and immunity Tde2/Tdi2

Released:
DOI: 10.2210/pdb8fzz/pdb
PDB entry 8fzz coloured by chain, front view.
PDB entry 8fzz coloured by chain, side view.
PDB entry 8fzz coloured by chain, top view.
Source organism: Phocaeicola vulgatus
Primary publication:
Structural disruption of Ntox15 nuclease effector domains by immunity proteins protects against type VI secretion system intoxication in Bacteroidales.
Bosch DE, Abbasian R, Parajuli B, Peterson SB, Mougous JD
OpenAccess logo mBio e0103923 (2023)
PMID: 37345922

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275551 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Novel toxin 15 domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 201 amino acids
Theoretical weight: 23.84 KDa
Source organism: Phocaeicola vulgatus
Expression system: Escherichia coli
UniProt:
  • Canonical: R9H4Y9 (Residues: 174-362; Coverage: 52%)
Gene name: C800_03411
Sequence domains: Novel toxin 15
DUF1851 domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 202 amino acids
Theoretical weight: 24.08 KDa
Source organism: Phocaeicola vulgatus
Expression system: Escherichia coli
UniProt:
  • Canonical: R9H4T6 (Residues: 1-196; Coverage: 100%)
Gene name: C800_03412
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 54.311Å b: 84.769Å c: 175.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.185 0.251
Expression system: Escherichia coli

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