› 8g0z

Electron Microscopy
3.61Å resolution

Mutant bacteriophage T4 gp41 helicase hexamer bound with single strand DNA and ATPgammaS in the stalled primosome

Released:
DOI: 10.2210/pdb8g0z/pdb
PDB entry 8g0z coloured by chain, front view.
PDB entry 8g0z coloured by chain, side view.
PDB entry 8g0z coloured by chain, top view.
Source organism: Escherichia phage T4
Primary publication:
Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Feng X, Spiering MM, de Luna Almeida Santos R, Benkovic SJ, Li H
Nat Commun 14 4396 (2023)
PMID: 37474605
Related structures: EMD-29658

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234960 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DnaB-like replicative helicase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 432 amino acids
Theoretical weight: 48.8 KDa
Source organism: Escherichia phage T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04530 (Residues: 1-432; Coverage: 91%)
Gene name: 41
Sequence domains: DnaB-like helicase C terminal domain
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: M
Molecule details ›
Chain: M
Length: 12 nucleotides
Theoretical weight: 3.61 KDa
Source organism: Escherichia phage T4
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand AGS 5 x AGS
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.61Å
Relevant EMDB volumes: EMD-29658
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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